Academic Dissertation Helsinki 2004 INTRODUCING WILSON DISEASE MUTATIONS INTO ZNTA Studies on the nucleotide and metal-binding sites of a bacterial zinc-translocating P-type ATPase

(2002) Introducing Wilson disease mutations into the zinc-transporting P-type ATPase of Escherichia coli. The mutation P634L in the " hinge " motif (GDGXNDXP) perturbs the formation of the E 2-P state. Eur. The nucleotide-binding domain of the Zn 2+-transporting P-type ATPase from Escherichia coli carries a glycine motif that may be involved in binding of ATP. Biochem. J. 377: 95-105 In addition, some unpublished data is included *) Equal contribution 5 Abbreviations A domain actuator domain ATPase adenosine triphosphatase cAPK cAMP-dependent protein kinase Cox cytochrome c oxidase GSH glutathione HAD haloacid dehalogenase IPTG isopropyl-β-D-thiogalactoside M domain membrane domain MBD metal-binding domain MNK Menkes disease protein N domain nucleotide-binding domain P domain phosphorylation domain P i inorganic phosphate PMSF phenylmethyl sulfonyl fluoride SDS sodium dodechyl sulphate SDS-PAGE sodium dodechyl sulphate polyacryl amide gel electrophoresis SOD superoxide dismutase TGN trans-Golgi network TM-helix transmembrane helix TNP-AMP trinitrophenyl adenosine monophosphate WND Wilson disease protein Wt wild-type 6